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VECTOR
INSERT
pET21b
MTD
~20 amino acid
Name : Clostridium botulinum neurotoxin type A light chain
Lc
Amino acid: 447 amino acid
Sequence : Clostridium botulinum type A Hall Lc
Tag
Hexa-histidine
OUR INGREDIENT
Structure of 'Methionyl r-Clostridium
Botulinum Polypeptide-1 Hexapeptide-40'
'Methionyl r-Clostridium Botulinum Polypeptide-1 Hexapeptide-40' is developed as recombination of peptide called “MTD” (Macro-molecule Transmission Domain) that has function of skin and target cell permeation and, active protein of botulinum toxin called 'Light Chain'. 'MTD' applied to our ingredient is originated and developed from botulinum toxin 'Heavy Chain', ,which performing a cell-penatration function of botulinum toxin.
STRUCTURE
MTD
Lc
His
469aa(53.6kDa)
COMPARISON IF BOTULINUM INGREDIENTS
Toxin Complex
(Medical Botox Injection)
Botulinum Light Chain A
Botulinum Heavy Chain
Hemaggultinin
Non-toxin
Non-toxin Non-Hemagglutinin
Composition
Heavy chain : Nerve cell penetration
Light chain : Botulinum Efficacy Part
Hemagglutinin
Non-toxin Non-Hemagglutinin
Commercialization
1980
Example
BOTOX (Allergan, US)
MEDITOXIN (Meditox, Korea)
Not permitted to use in Cosmetic Product
Molecular weight
900KDa
Pure Toxin
(Medical Botox Injection)
Botulinum Light Chain A
Botulinum Heavy Chain
Composition
Heavy chain : Nerve cell penetration
Light chain : Botulinum Efficacy Part
Commercialization
1995
Example
XEOMIN (Merz, Germany)
Not permitted to use in Cosmetic Product
Molecular weight
150KDa
Our Ingredient
(BPMed_BoLCA_Protein)
Botulinum Light Chain A
MTD Protein
Composition
Light Chain + MTD
Botulinum Efficacy Part
Marco-molecule Transmission Domain
(Skin & Nerve cell penetration)
= Recombination of the Protein
Commercialization
2014
Example
BoLCA+ (BPMed cosmetic, Korea)
The First Botulinum Ingredient on ICID
BPMed Cosmetic has Exclusivity
Molecular weight
50KDa
SKIN AND CELL PENETRATION TECHNOLOGY OF MTD
Platform technology to improve transdermal delivery of
'Methionyl r-Clostridium Botulinum Polypeptide-1 Hexapeptide-40'
1st Action
Improved penetration of functional materials into stratum corneum and other skin layers by MTD.
2nd Action
Maximized efficacy through the delivery of 'Methionyl r-Clostridium Botulinum Polypeptide-1 Hexapeptide-40' into specific targets.
Epidermis
Dermis
Subcutaneous Tissue
Muscle Layer
Cell
Modality of Intracellular delivery of MTD in 'Methionyl
r-Clostridium Botulinum Polypeptide-1 Hexapeptide-40'
Permeate in to cell by MTD technology shows very high Permeability and significantly less cell damage then nano liposome or common peptide ingredients(PTD).
MECHANISM ON NERVE CELL
Normal Neurotransmission Process
SNARE Complex Exocitosis Acetylcholine Diffusion
Binds to the Receptors on the Muscle
Nerve Cell
Approaching to the Cell
SNAP 25
Moves to the SNAP25
Penetrated in to dermis layer
Methionyl r-Clostridium Botulinum
Polypeptide-1 Hexapeptide-40
Direct Cell Membrane Translocation
Cleaves SNAP25 by Light Chain Part